Journal: Frontiers in Neurology

Article Title: KCNB1 mutation impairs neuronal differentiation by disrupting gene expression temporal regulation and neuron-specific pathways

doi: 10.3389/fneur.2026.1739214

Figure Lengend Snippet: Induced differentiation of iPSCs. (A) Experimental schematic of the iPSC differentiation process. (B) Representative morphological images of healthy donor-derived NPCs at neuron day 5 and forebrain precursors at neuron day 10. (C) Representative images of immunofluorescence-stained neurons derived from the healthy donor. Neurons were stained with MAP2 (green) and NeuN (red). Nuclei were stained with DAPI (blue). Scale bar: 10 μm. (D) Representative morphological images of patient-derived NPCs at neuron day 5 and forebrain precursors at neuron day 10. (E) Representative images of immunofluorescence-stained neurons derived from the patient. Neurons were stained with MAP2 (green) and NeuN (red). Nuclei were stained with DAPI (blue). Scale bar: 10 μm. BDNF, Brain-Derived Neurotrophic Factor; GDNF, Glial Cell Line-Derived Neurotrophic Factor; LAA, L-Ascorbic acid.

Article Snippet: Recombinant Human/Murine/Rat BDNF , Novoprotein , C076.

Techniques: Derivative Assay, Immunofluorescence, Staining

Journal: Scientific Reports

Article Title: A comparison of commercial assays quantifying mature brain-derived neurotrophic factor (mBDNF) and its precursor (pro-BDNF) in human serum

doi: 10.1038/s41598-025-22278-7

Figure Lengend Snippet: Levels of proteins (ng/mL) obtained in human serum samples ( n = 23). Different dilutions were assessed in ELISA kits from R&D Systems ( A , B and E ) [total BDNF Quantikine ELISA kit#DBNT00 (A) , Human pro-BDNF DuoSet ELISA kit #DY3175 (B) and human free BDNF Quantikine ELISA kit #DBD00 (E) ], Aviscera Bioscience ( C and F ) [high sensitivity pro-BDNF human ELISA kit #SK00752-09 (C) and high sensitive BDNF ELISA kit #SK00752-01 (F) ] and Finetest ( D and G ) [human pro-BDNF ELISA kit #EH4255 (D) and human BDNF ELISA kit #EH0043 (G) ]. Comparison of protein levels obtained in human serum samples diluted at 1:20 (H) for pro-BDNF and at 1:40 (I) for BDNF (common dilutions) in the ELISA kits from the 3 companies used in this study (R&D Systems, Aviscera Bioscience and FineTest). Each value obtained of the samples analysed was represented, and the median ± SD of all of them.

Article Snippet: In the case of the total BDNF and human free BDNF Quantikine ELISA kits (R&D Systems; #DBNT00 and #DBD00, respectively), primary antibodies were HRP-conjugated, eliminating the need for further detection with a secondary antibody.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Scientific Reports

Article Title: A comparison of commercial assays quantifying mature brain-derived neurotrophic factor (mBDNF) and its precursor (pro-BDNF) in human serum

doi: 10.1038/s41598-025-22278-7

Figure Lengend Snippet: Western blot for qualitative analysis of pro-BDNF and mBDNF antibodies. Antibodies from R&D Systems [total BDNF Quantikine #DBNT00 (A) , human pro-BDNF DuoSet #DY3175 (B) and human free BDNF Quantikine #DBD00 (C) ], FineTest [human pro-BDNF #EH4255 (D) and human BDNF #EH0043 (E) ], and Aviscera Bioscience [high sensitivity pro-BDNF human #SK00752-09 (F) and high sensitive BDNF #SK00752-01 (G) ] ELISA kit were tested. Original blots are presented in Supplementary Figure S6.

Article Snippet: In the case of the total BDNF and human free BDNF Quantikine ELISA kits (R&D Systems; #DBNT00 and #DBD00, respectively), primary antibodies were HRP-conjugated, eliminating the need for further detection with a secondary antibody.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay